Critical Aspects of Detection of Sperm DNA Fragmentation by Tunel/Flow Cytometry

Clinical aspects of sperm DNA fragmentation detection and male infertility.

Over the past 25 years, various methods have been developed to measure sperm DNA strand breaks in situ. Currently, there are four major tests of sperm DNA fragmentation, including the Comet, Tunel, sperm chromatin structure assay (SCSA) and the acridine orange test (AOT). The Comet assay is a light microscope technique where the sperm cells are mixed with melted agarose and then placed on a gla...

Sperm DNA fragmentation: laboratory and clinical aspects

extremal region detection guided by maxima of gradient magnitude

a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...

Human sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy.

An association between DNA fragmentation in sperm determined by the terminal deoxynucleotidyl transferase [TdT]-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) assay and the incidence of reproductive failure has been reported, either using flow cytometry or optical microscopy. However, the results obtained using each of these two approaches are different. Since there is a re...

Sperm DNA fragmentation

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. It is an honour to write the foreword to this focused issue of TAU on sperm DNA fragmentation (SDF). From my time as a first generation embryologist in the early days at the University of Cambridge with Professor Robert Edwards and in my current role at the London Women’s Clinic, I have become acutely aware of the num...